利用调制荧光仪在线监测叶绿素荧光

In situ monitoring of chlorophyll fluorescence using PAM fluorometer

介绍了利用便携式调制荧光仪PAM-2100在线监测叶绿素荧光的技术。该技术不影响植物的自然光合状态, 可以在线监测Ft、Fm'、Y、rETR、qP、qN或NPQ、PAR和叶温等指标。以凤眼莲为例进行了在线监测,每隔5 min 监测一次,共进行了225 min的监测。结果表明叶绿素荧光参数的变化依赖于PAR的变化。Ft、rETR、qN和NPQ的变化与PAR的变化趋势一致,Fm'、Y和qP的变化与PAR的变化相反。通过对凤眼莲的在线监测,说明该技术是可靠的,具有简单、快速、灵敏等特点。随着新型调制荧光仪的出现,该技术可能在植物生态学领域得到广泛应用。

This paper introduced an in situ monitoring technique of chlorophyll fluorescence using portable Pulse-Amplitude Modulation fluorometer, PAM-2100. With this technique Ft, F′m, Y, rETR, qP, qN or NPQ, PAR, and leaf temperature can be monitored without disturbing plant natural photosynthesis. An in situ monitoring case with Eiehhornia crassipes was examined. Chlorophyll fluorescence were measured every 5 min and monitored continuously for 225 rain. The results indicated that the changes of chlorophyll fluorescence parameters depended on the variation of environmental PAR. Some parameters such as Ft, rETR, qN and NPQ showed a consistent change with PAR, while F′m, Y and qP changed reversely. This case demonstrated that in situ monitoring technique was reliable, simple, rapid and sensitive. Along with the appearance of new modulated fluorometers suit for in situ monitoring, this technique may be widely used in plant ecology research.