间变性星形细胞瘤中DR4及DR5的表达

Expression of DR4 and DR5 in human anaplastic astrocytoma

目的研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)的死亡受体(death receptor,DR)DR4和DR5在间变性星形细胞瘤中的表达,并探讨其临床意义。方法联合采用免疫组化和原位杂交方法检测24间变性星形细胞瘤和16例正常脑组织中DR的表达。结果免疫组化染色显示,24例间变性星形细胞瘤均大量表达死亡受体DR4和DR5,而16例正常脑组织中7例(43.8%)表达DR4,5例(31.3%)表达DR5。间变性星形细胞瘤组织中DR蛋白的表达显著高于正常脑组织中DR蛋白的表达,两者差异有显著性(P<0.01)。原位杂交显示,DR在全部24例间变性星形细胞瘤组织和大部分正常脑组织中均呈强阳性表达,二者差异无显著性(P>0.05)。结论间变性星形细胞瘤细胞中普遍存在DR的高表达,这可能为间变性星形细胞瘤的凋亡诱导治疗提供新的靶点。DR蛋白在正常脑组织和间变性星形细胞瘤中的表达差异,可能是TRAIL选择性诱导凋亡的机制之一。

Objective To investigate the expression and significance of DR4 and DR5 in human anaplastic astrocytoma. Methods The expression of DR4 and DR5 was determined by immanohistochemistry and in situ hybridization in 24 samples of anaplastic astrocytoma and 16 samples of normal brain tissue. Results DR was expressed highly in all anaplastic astrocytoma samples, while its expression in part of normal brain tissue was low. DR protein expression in anaplastic astrocytoma tissue was significantly higher than that in normal brain tissue （ P 〈 0.01） .There was no significant difference between the expression of DR mRNA in normal brain tissue and that in anaplastic astrocytoma tissue（ P 〉 0.05）. Conclusions High DR expression is prevalent in anaplastic astrocytoma tissue and this may contribute to the apoptosis-inducing therapy of anaplastic astrocytoma. The difference in expression of DR protein in normal brain tissue and in anaplastic astrocytoma may be one of the mechamisms of selective induction of apoptosis by TRAIL.