灭幼脲对云斑天牛的不育作用及核酸含量的影响

STERILE ACTIVITIES OF DIFLUBENZURON AGAINST BATOCERA HORSFIELDI (HOPE) AND EFFECTS ON DNA, AND RNA CONTENTS OF OVARIES, EGGS AND EMBRYO

用灭幼脲处理的补充营养寄主植物饲养云斑天牛Ｂａｔｏｃｅｒａｈｕｒｓｆｉｅｌｄｉ（Ｈｏｐｅ）成虫，对子代卵的孵化有明显抑制作用。生化分析表明：灭幼脲可以促进雌虫卵巢组织ＤＮＡ、ＲＮＡ及一日龄卵ＤＮＡ的合成；对一日龄卵内ＲＮＡ含量影响不显著；对发育末期胚胎的ＤＮＡ、ＲＮＡ有明显抑制作用。结合灭幼脲在世代间垂直传递途径，讨论了灭幼脲对云斑天牛成虫不育作用的机理。

When adults of Batocera horsfieldi (Hope) were fed continuously with the adult host plant treated by different concentrations of diflubenzuron, the hatching of coming generation eggs was severely inhibited. The biochemistry analysis showed that both DNA, RNA content in the ovaries and DNA content in the ten hour old eggs increased with the diflubenzuron treatment. But the RNA content in the ten hour old eggs was a little affected. In addition, DNA and RNA contents at the last stage of embryonic development obviously decreased. Concerning its vertical transferring pathway between the generations, mechanism of action of sterilization by diflubenzuron on the adult of B.horsfieldi (Hope) was discussed.