糖肾宁对糖尿病肾病大鼠脂质代谢及氧自由基的影响

Effect of “Tang Shen Ning” On Lipidic Metabolism and Oxygen-Derived Free Radicals In Rat Model of Diabetic Nephropathy

目的探讨中药复方糖肾宁对糖尿病肾病(diabeticnephropathy,DN)大鼠脂质代谢和氧化抗氧化系统的影响。方法高脂饲料喂养加腹腔注射链脲佐菌素(streptozotocin,STZ)建立DN模型,按24h尿微量白蛋白(24hUA1b)排泄量分为模型组、蒙诺组、糖肾宁组,分别予相应药物灌胃,8周后测定24hUA1b,血胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL),空腹血糖(FBS)、血清胰岛素(FINS),并计算胰岛素敏感指数(ISI)。肾组织匀浆测超氧化物歧化酶(SOD)、丙二醛(MDA),游离脂肪酸(NEFA)。结果糖肾宁组大鼠24hUA1b、FBS、FINS、LDL和肾组织NEFA、MDA较模型组降低,ISI和肾组织SOD升高,TG和SOD与对照组比较也有显著差异。结论糖肾宁可减少DN大鼠24hUA1b,其机制与改善糖和脂质代谢,调节氧化抗氧化系统有关。

Objective To study the effect of “Tang Shen Ning” on lipidic metabolism and oxygen-derived free radicals in rat model of diabetic nephropathy. Methods Male Wistar rats were randomly divided into the normal group（fed with normal diet）and model group（fed with high caloric diet）. Ten weeks later intraperitoneal injection of streptozotocin（STZ） was applied, and a week later, the model group rats were randomly divided into pathologic group, Monopril group and TSNR group according to the 24hU-A 1 b. Each group＇ s 24 Hu-A 1 b ,TC ,TG, HDL, LDL, FBS, FINS, ISI and SOD, MDA, NEFA in the rat＇s renal tissue were determined. Results The levels of FBS, FINS,24h U-A1b,TG , LDL,MDA,NEFA in TSNP group were significantly lower than those in pathologic group,and SOD, ISI increased obviously. There are significant differences in TC and SOD between control group and treatment group. Conclusion The results indicate that “Tang Shen Ning” can lower the micro-albuminuria in DN rats, the mechanism of which probably lies in the modification of glycol metabolism,increasing the ISI, and reducing blood lipids.