摘要
目的:探讨巨型辐花海葵荧光蛋白类似物基因hmGFP的生物学特性及应用的可能性。方法:构建了hmGFP的两个原核表达载体并进行诱导表达。结果:低温下hmGFP获得了功能性表达而发光。深入比较了重组巨型辐花海葵荧光蛋白类似物基因hmGFP在两个表达体系中的表达差异,其中pETTRX-hmGFP的重组蛋白表达水平和可溶性均比pET21b-hmGFP高。结论:对pETTRX-hmGFP的表达条件进行了优化,为下一步hmGFP成熟重组蛋白的纯化及其荧光波谱分析奠定了良好基础。
Objective：To gain insight of biological characteristics and application feasibility for GFP homolog hmGFP from Heteractis magnifica. Methods： Two different prokaryotie expression systems （ pET21 b and pETTRX） of hmGFP were constructed and recombinant hmGFP can be both functionally expressed under low temperature. The expression difference between pET21b-hmGFP and pETTRX-hmGFP were further investigated. Results：The expression level and the solubility of the recombinant hmGFP protein in pETTRX were better than that in pET21b. Conclusion： The optimal pETTRX-hmGFP expression condition was established for next step of purifying mature recombinant hmGFP protein to characterize its fluorescent spectrum.
出处
《广州医学院学报》
2005年第3期1-6,共6页
Academic Journal of Guangzhou Medical College
基金
广州医学院博士启动基金(025105)
