幽门螺杆菌感染与老年消化性溃疡发病关系的调查

The Relationship between Helicobacter Pylori Infection and Pathogenesis of Peptic Ulcer in the Elderly

目的:探讨幽门螺杆菌(HP)阳性和阴性老年消化性溃疡病人胃窦部生长抑素、胃泌素及D、G细胞密度的关系.方法:2000年2月～2004年9月在本院内科住院病人80例,均经胃镜距幽门5 cm处胃窦部取黏膜组织8块.3块进行快速尿素酶试验(UT试验),Worenin-starry银染色及HE组织病理学检查,2块活检胃组织用待测生长抑素及胃泌素,余3块组织用缓冲液及Boriv液固定待进行胃窦黏膜D、G细胞免疫组化染色.结果:HP阳性组较HP阴性组生长抑素含量明显减少(P＜0.01),胃泌素/生长抑素比值明显增加(P＜0.05);D、G细胞密度明显降低(P＜0.01),G/D比值明显升高(P＜0.05).结论:HP感染在老年消化性溃疡发病中起重要作用.

Objective： To explore the result of Helicobacter pylori （HP） test in relation to the levels of somatostatin, gastrin and densities of D and G cells at the gastric antrum in elderly patients with peptic ulcer. Methods： 8 specimens of antral mucosa 5 cm to the pylorus were obtained using endoscopy from each of all eases studied. Of the 8 specimens, 3 were tested by rapid urokinase test, and observed for pathology using Worenin-Starry and HE staining; 2 were determined for somatostatin and gastrin levels; and the other 3 detected for antrum mucosal D and g cells using immunohistochemistry after treated with buffer and Boriv fixation solution. Results： compared with Hp-negative group, tip-positive group showed significantly lower level of somatostatin （P 〈 0.01 ） , markedly elevated ratio of gastrin over somatostatin （P 〈 0.05） , lower densities of D and C cells （P 〈0.01 ） and higher C/D ration （P 〈0.05）. Conclusion： Hp infection may be playing an important role in the pathogenesis of peptic ulcer in the elderly.