摘要
目的了解呼吸道感染患儿肺炎支原体(M P)、解脲脲原体(U u)、沙眼衣原体(CT)的感染情况。方法应用荧光定量PCR(FQ-PCR)方法检测351例呼吸道感染患儿血清M P、U u、CT。结果M P、U u、CT总检出率为58.4%,其中M P感染占29.9%;U u为21.3%;CT为7.4%;M P和U u混合感染率为3.2%;U u和CT混合感染率为1.4%。在年龄分布上,新生儿组患儿U u感染率为30.3%,与其他组比较差异有显著性;但M P感染率显著低于其他年龄组。M P、U u为冬春季感染率高;CT春季感染率低。M P、U u在痰标本中的检出率高于血标本。结论新生儿较其他年龄组患儿易感染U u、CT,M P随年龄增长感染率逐渐增高。痰标本的阳性率显著高于血清。荧光定量PCR法诊断支原体、衣原体感染快速而敏感,特异性高。
Objective To investigate the distribution of infection of mycoplasma pneumoniae (MP),ureaplama urealyticum (Uu) and chlamydia trachomatis (CT) in children with respiratory tract infection. Methods MP,Uu and CT were measured simultaneously by fluorogenic quantitative PCR in 351 children with respiratory tract infection. Result Total infectious rate of MP ,Uu and CT was 58.4%. Among them, MP was 29. 9%, Uu was 21.3 %, CT was 7.40%. The mixed infectious rate of MP and Uu was 3.2%, Uu and CT was 1.4%. The group whose age under twenty-eight days had a higher positive rates of Un and CT,and a lower positive rate of Mp. The infectious rates of MP were not significant difference in faur seasons (P〉0.05), MP and Uu were higher in winter and spring,and CT was lower in spring. There were significant difference in MP and Uu between serum and sputum samples (P〈0. 05). Conclusion Neonates had susceptibility to Uu and CT; infectious rate of MP were increased with age. Positive rate of sputum speciments were higher than those of serum. Fluorogenic quantitative PCR was sensitive ,specific and rapid to increase the diagnosis of MP ,Uu and CT infections.
出处
《临床输血与检验》
CAS
2005年第4期259-261,共3页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
呼吸道感染
肺炎支原体
解脲脲原体
沙眼衣原体
荧光定量PCR法
Respiratory tract infection Mycoplasma pneumoniae Ureaplama urealyticum Chlamydia trachomatis Fluorogenic quantitative PCR