用非常规培养方法提高乳酸菌产酸率的研究

Enhanced lactic acid production by LAB using non-conventional cultivation

以德氏乳酸杆菌为研究对象,考察了八种有机溶剂分别加入培养基对细菌生长及产酸的影响。结果表明,采用油醇和三辛胺混合溶剂时,既能降低对细胞生长的毒性,又保持了较强的萃取能力。对悬浮细胞发酵和萃取整合的方法和固定化细胞发酵和萃取整合的方法进行了比较,表明这两种方法均较常规培养方法提高乳酸产率60%以上。

Biocompatibility of eight organic solvents for Lactobacillus delbruckii was investigated and the mixture of oleyl alcohol and trioctylamine was selected as the solvent of extractive cultivation. Two methods using extractive fermentation were compared. The first, cells were free. The second, cells were immobilized by Ca-alginate gel bead. Both methods produced approximate more than 60 % lactic acid compared with that of the control.