高效液相色谱法测定保留灌肠液中3成分的含量

Determination of furazolidone, trimethoprim and prednisone acetate in enema by HPLC

目的:建立同时测定灌肠液中呋喃唑酮、甲氧苄啶和醋酸泼尼松3种成分含量的方法.方法:流动相:甲醇-水(用醋酸和氨水调至pH 6.6)(60:40);流速:1 mL·min-1;检测波长:231 nm.结果:呋喃唑酮在10～50mg·L-1,甲氧苄啶在10～50 mg·L-1,醋酸泼尼松在2～10mg·L-1范围内线性关系良好,回收率试验结果满意,重复性好.结论:本法简便、准确,可以用于制剂质量控制.

OBJECTIVE To establish an HPLC method for determination of furazolidone, trimethoprim and prednisone acetate in enema. METHODS Mobile phase of methanol-water（adjust pH to 6. 6 using acetate acid or ammonia solution） （60：40） at the flow rate of 1 mL·min^-1 and the wavelength of detection was at 231 nm, RESULTS In this condition there was a good linear relationship over the concentration range of 10 - 50mg· L^-1 for furazolidone, 10 - 50 mg·L^-1 for trimethoprim,2 - 10mg·L^-1 for prednisone acetate. CONCLUSION This method is simple and accurate, it could be applied to the determination of the enema.