鸡FABP基因多态性研究

Study on the FABP Gene Polymorphism of Six Chicken Breeds

采用PCR-RFLP方法对金水乌鸡、武定鸡、湖北红鸡、青脚麻鸡、矮黄鸡和隐性白羽肉鸡6个品种共计360只鸡的FABP基因进行多态性分析,选用HhaⅠ、HaeⅢ、HinfⅠ、BamHⅠ、EcoRⅠ和PstⅠ6种限制性内切酶对PCR产物进行单酶切,结果发现HhaⅠ-RFLP具有多态性,有AA和AB两种基因型,该基因座频率分布经独立性χ2检验,结果表明,蛋用品种湖北红鸡与其他品种间存在显著或极显著差异,群体中B基因频率占明显优势;矮黄鸡与隐性白羽肉鸡2个肉用品种鸡与其他品种间也存在显著或极显著差异,这2个品种的优势基因为A基因。

In this article,the polymerase chain reaction-restietion fragment length polymorphisms （PCR-RFLPs）of FABP gene in 360 chickens from 6 chickens breeds including Jinshui silk ehieken,Wuding chieken,Hubei red egg chieken,Qinjiaoma chicken,orange dwarf chicken and white recessive chicken were investigated. Six restrictions of Hha Ⅰ ,Hoe Ⅲ ,Hinf Ⅰ ,Barn H Ⅰ ,Eco R Ⅰ and Pst Ⅰ were used to digeste the production of PCR respectively.It was demonstrated that difterent genotypes （AA and AB） of FABP gene had been detected for Hha Ⅰ site.The result of Chi Square（x^2） test showed that the difference of genetype frequency between Hubei red egg chicken,orange dwarf chicken or white reeessive chicken and the other 5 chicken breeds was significant （P〈0.05） or greatly significant （P〈0.01）.The B gene was the superior gene in Hubei red egg chicken and in the two broiler chicken breeds. The frequency of A gene was greatly higher than B gene.