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小单孢菌40027菌株原生质体的形成和再生 被引量:2

Formation and Regeneration of Protoplasts in Micromonospora sp. 40027
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摘要 通过酶解温度、酶解时间和再生培养基对小单孢菌40027菌株原生质体形成和再生的研究,确定小单孢菌40027菌株原生质体制备条件为37℃酶解60min;原生质体的再生培养基为R-1培养基。 The effects of lytic temperature,lytie time and reaeneration medium on the formation and regeneration of protoplasts in Micromonospora sp.40027 were studied.The result showed that optimal lytic temperature and lytic time were 37℃ and 60mins. and optimal regeneration medium was R-1.
出处 《湖北农业科学》 北大核心 2005年第5期61-62,107,共3页 Hubei Agricultural Sciences
基金 湖北省自然科学基金项目(2004ABA129) 中南民族大学自然科学基金重点项目(YZZ04003)。
关键词 小单孢菌 原生质体 再生 Micromonospora protoplasts regeneration
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