摘要
动物组织中残留的阿维菌素、依维菌素和多拉菌素经乙腈提取,碱性氧化铝固相萃取柱净化后,与荧光衍生化试剂反应,产物用高效液相色谱(荧光检测器)进行检测,实现了阿维菌素类药物的残留分析.本方法在猪组织中测定阿维菌素类药物的检测限为1μg/kg;在猪组织中添加浓度为1、2、5μg/kg时的回收率为55%～75%.
A multi-residue analytical method has been developed for the quantitative determination of avermectin （AVM）, ivermectin （IVM） and doramectin （DRM） in swine tissue samples. The samples are extracted with acetonitrile, followed by clean-up on a alumina column, extracts are derivatied and determined by high performance liquid chromatography （HPLC） with fluorescence detection. Recoveries ranged from 55 % to 75 % for swine tissue samples fortified with avermectins ranged from 1 -5 μg/kg. The limit of detection is 1 μg/kg.
出处
《中国兽药杂志》
2005年第9期12-15,共4页
Chinese Journal of Veterinary Drug
基金
国家科技攻关项目(2002BA906A75)
关键词
高效液相色谱
阿维菌素
荧光
残留
solid phase extraction
high performance liquid chromatography （ HPLC ）
avermectins
residue
swine itssue
