茂原链霉菌谷氨酰胺转胺酶序列分析及三维结构建模

Sequence analysis and three-dimensional structure modeling of transglutaminase from streptomyces mobaraensis

利用DNASTAR、VectorNTI9、SignalP等生物信息学平台对茂原链霉菌(Streptomyces mobaraensis)谷氨酰胺转胺酶(TGase)进行了序列分析和三维结构建模。结果表明:茂原链霉菌TGase同已报道其他微生物来源的TGase有较高的同源性,无信号肽,存在一个跨膜结构区,二级结构是由多个α螺旋、转角和少量β折叠构成。同时在一二级结构分析的基础上,利用同源建模的方法完成了三维结构的建模。

Sequence analysis of Transglutaminase from streptomyces mobaraensis was conducted by bioinformatic tools such as DNAstar, Vector NTI and SignalP etc.. The results show that the TGase obtained from S, mobaraensis in this study had higher similarity with reported microbial TGase, contained a transmemberane regions but no signal peptide and its secondary structure was composed of many alpha regions, turns regions and few beta regions. Based on the primary and secondary structure analysis of TGase, Three - dimensional structure of TGase was developed by homology modeling methods.