摘要
目的:观察清热利湿中药对肝细胞膜ATP酶活性的影响。探讨其防治结石形成的机理。方法:采用肝脏原位灌注消化法体外培养大鼠肝细胞,以及血清药理学方法,测定肝细胞膜Ca2+-Mg2+-ATPase、Na+-K+-ATPase的活性。结果:10倍量和20倍量载药(清热利湿中药)血清能提高炎性细胞因子刺激后Ca2+-Mg2+-ATPase、Na+-K+-ATPase活性。结论:清热利湿中药可能是通过提高肝细胞膜ATP酶活性,减轻细胞内钙超载,降低胆汁的成石倾向。
Objective :To observe the effect of QingReLiShi herbs to the activity of ATPase of liver cell membrane, to evaluate the pathogenesis of preventing the formation of pigment stones. Methods : We adopt the rat hepatic cells by using primary lives infusion method and serum pharmacology method, determine th activity of Ca^2+ - Mg^2+ - ATPase and Na^+ - K^+ -ATPase of liver cell membrane. Results:lO times and 20 times dose herb serum can improve the activity of Ca^2+ - Mg^2+ - ATPase and Na^+ - K^+ - ATPase after the incetive of the inflammatory factors. Conclusion :QingReLiShi herbs decreased the tendency of glasstone by increasing the activity of ATPase of liver cell membrance, relieving calcium overload in cells possibly.
出处
《中医药学刊》
2005年第9期1605-1607,共3页
Study Journal of Traditional Chinese Medicine
基金
天津市科委课题