谷氨酰胺对烧伤后大鼠肠道损伤及炎症反应的影响

Effects of Glutamine on Intestine Damage and Inflammatory Reaction after Severe Burn Injury

目的探讨谷氨酰胺对烧伤后大鼠肠道损伤及炎症反应的影响。方法采用30％体表面积Ⅲ°烧伤大鼠模型,将88只Wistar大鼠随机分为烧伤对照组和烧伤后补充谷氨酰胺(GLN)组。两组大鼠采用等氮、等热量的营养支持,GIN组每天给予1.0 g／kg GLN,对照组给予等量的甘氨酸。观察烧伤前及烧伤后第1、3、5、7、10天大鼠静息能量消耗(REE)的变化,同时检测烧伤后血浆二胺氧化酶(DAO)活性及内毒素(LPS)、肿瘤坏死因子(TNF)和白细胞介素-1(IL-1)的含量。结果烧伤后两组大鼠的REE、DAO、TNF、LPS和IL-1均明显高于烧伤前(P＜0.01),GLN组REE、血浆DAO活性及TNF、LPS和IL-1含量均明显低于对照组(P＜0.05,P＜0.01)。结论口服GLN能减轻烧伤后肠道受损程度,降低烧伤大鼠血中炎症介质水平,从而降低伤后机体高代谢反应。

To study the effect of glutamine on intestine damage and inflammatory reaction after severe burn injury. Methods Eighty-eight Wistar rats were inflicted with 30% total body surface area full thickness burns and then randomly divided into burned control （B） group and glutamine treatment （GLN） group. Isonitrogenous and Isocaloric nutrition supports were provided to both two groups. Rats in GLN group and B group were administered with glutamine or glycine at 1.0 g/（kg ·d）, respectively. Resting energy expenditure （REE）, plasma DAO, and the levels of plasma TNF, LPS, and IL-1 were determined. Results After burn injury, the REE, DAO activity, and levels of TNF, LPS, and IL-1 significantly increased. Comparel with B group, REE, plasma DAO activity and levels of TNF, LPS, and IL-1 in GLN group were significantly lower. Conclusion Oral glutamine administration may relieve intestine damage after severe burn injury, lower intestinal mucosal permeability, and reduce the hypermetabolic reaction.