神经酰胺对小鼠皮层神经元乳酸脱氢酶代谢的影响

The Effect of Ceramide on Lactate Dehydrogenase Metabolism of Primary Cultured Mice Cortical Neurons

目的研究神经酰胺对小鼠皮层神经元乳酸脱氢酶(LDH)代谢的影响。方法在培养的小鼠皮层神经元上清中分别加入50、100、200、500、1000、2000nmol/L的神经酰胺,分别作用0、1、4、8、12、16、24、36h,测定LDH浓度,计算其代谢率和漏出率。结果可显著改变乳酸脱氢酶(LDH)在细胞内外的分布,随神经酰胺作用时间的延长或剂量的增加,细胞外LDH含量显著升高,但神经酰胺对细胞总LDH代谢无影响。结论神经酰胺可增加LDH漏出率,但对细胞总LDH代谢无影响。

Objective To explore whether natural ceramide has effect on lactate dehydrogenase （LDH） metabolism in primary cultured mice cortical neurons. Methods Ceramide at different concentrations of 50,100,200,500, 1000,2000 nmol/L was given to the culture medium of primary cuhured mice cortical neurons. The LDH concentration was assayed after 0 h, 1 h,4 h, 8 h, 12 h, 16 h,24 h,36 h respectively. Its metabolism rate and leakage rate were calculated respectively. Results Ceramide changed significantly the distribution of LDH inside and outside of the neurons. Exogenous ceramide stimulated LDH elevation in supernatant in a dose-dependent and time-dependent manner, but had little impact on the total LDH metabolism of neurons. Conclusion Ceramide elevates the leakage rate of LDH and has no effect on total LDH metabolism of neurons.