摘要
将猪肌生成抑制素成熟蛋白编码序列真核表达质粒,采用脂质体法转染COS-7细胞,经RT-PCR检测,转染重组质粒的COS-7细胞MSTN mRNA呈阳性,表明该重组体能够在真核细胞中完成转录过程。SDS-PAGE电泳结果有43 ku目的蛋白表达带,并用Western blotting证实了其表达的特异性,表明猪肌生成抑制素成熟蛋白编码序列可以在真核细胞中表达。
In this paper, recombinant plasmid pcDNA3. 1-mpMSTN was introduced into the cell of COS-7 by lipofectamineTM. After 68 h,the expression of myostatin mature protein coding sequences was detected by RT-PCR. It was also detected by SDS-PAGE and Western blotting assay. The results show that recombinant plasmid of pcDNA3.1-mpMSTN has a special band of 43 ku.
出处
《动物医学进展》
CSCD
2005年第9期59-61,共3页
Progress In Veterinary Medicine
基金
国家自然科学基金资助项目(30070563)
