HAP纳米粒子进入癌细胞的体外实验研究

The Study of HAP Nanoparticles Uptaken by Cells in Vitro

目的验证羟基磷灰石(HAP)纳米粒子与癌细胞共同孵育后,细胞质内发现的大量颗粒物质,是HAP纳米粒子,以证实HAP纳米粒子能以纳米粒子形式进入癌细胞。为进一步深入探讨HAP纳米粒子在癌细胞内的代谢过程提供实验依据。方法将BEL7402肝癌细胞与HAP纳米粒子共同孵育8h后,应用透射电子显微镜观察,并进行了能谱检测和电子衍射图谱分析。结果透射电镜观察到癌细胞内外均有片状的颗粒物质;能谱显示这些颗粒状物质为含Ca、P的粒子;电子衍射图谱测试表明其d值符合HAP晶体的特征。结论进入到肝癌细胞内的颗粒物质即为HAP纳米粒子,说明HAP纳米粒子能以纳米颗粒的形式进入肝癌细胞。

Object Recently, many particles were found in the cytoplasm area after the incubation of hydroxyapatite（HAP） nanoparticles and cultured hepatoma cells. The purpose of this study was to confirm whether the particles in the cytoplasm of hepatoma cells were HAP nanoparticles exactly. Method BELT402 cancer cell were incubated with HAP nanoparticle Sol for 8 hours. Then, cells were collected for specimen preparation. Transmission Electron Microscope （TEM）, energy dispersing spectrum （EDS）and electronic diffraction （ED）attached to TEM were used to detect the properties of the particles. Result Many particles similar to HAP in shape are found in the cytoplasm under TEM. By EDS analysis, they are the particles containing calcium（Ca） and phosphorus （P）. The classic tings of HAP crystal appear in the E D pictures of these particles. Conclusion Thus, the particles in cytoplasm are confirmed as HAP nanoparticles exactly. This indicates that HAP nanoparticles could be absorbed by cells as the entire crystal particles.