摘要
目的探讨热应激时内皮细胞与白细胞黏附的改变及油茶皂苷对此影响。方法建立人脐静脉内皮细胞(HU-VECs)热应激损伤模型,以油茶皂苷(10.0,1.0,0.1μmol.L-1)预处理细胞5 h,取培养细胞上清液测定LDH活性,HUVECs分别用于测定细胞存活率,白细胞黏附率及细胞间黏附分子-1(ICAM-1)的表达。结果热应激可引起LDH活性升高,白细胞黏附率及ICAM-1表达的增加,与对照组比较,差异具有显著性(P<0.01),但细胞存活率无明显下降。油茶皂苷呈浓度依赖性地对抗上述改变,但对细胞存活率无明显影响。结论油茶皂苷可抑制热应激诱导内皮细胞与白细胞黏附的增加,其机制可能是下调ICAM-1的表达。
Aim The present study focused on the changes of leukocyte adhesion to endothelial cells and the effects of sasanquasaponin （ SQS ） on these changes. Methods After pretreated by SQS （ 10. 0, 1.0, 0. 1μmol·L^-1） for 5 hous, human umbilical vein endothelial cells （HUVECs） were exposed to heatstress for 3 hours. Lactate dehydrogenase （ LDH ） activity in the HUVECs supematants was determined. The resulting HUVECs viability and leukocyte adhesion rate were measured spectrophotometrically. The expression of ICAM-1 was assayed by western blots. Results Elevation of LDH activity, enhancement of leukocyte adhesion rate and ICAM-1 expression level were observed in heat-stress group, and there were significant differences compared with control group （P 〈 0. 01 ） ; however, there was no marked effect of heatstress on cell viability. Inversely, SQS antagonized these changes in a concentration-dependent manner while exerted no effect on cell viability. Conclusion SQS can inhibit the enhancement of leukocyte adhesion to HUVECs induced by heat-stress and its mechanism is likely to downregulate ICAM-1 expression.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第9期1092-1095,共4页
Chinese Pharmacological Bulletin
基金
江西省自然科学基金资助项目(No0140030)
