阿朴吗啡诱导黑质毁损大鼠纹状体c-fos表达被引量：1

Apomorphine induced c-fos expression in the striatum of 6-OHDA-lesioned rats

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摘要目的观察6-羟基多巴胺(6-OHDA)毁损黑质后,不同时间点腹腔注射阿朴吗啡(APO)大鼠纹状体c-fos表达情况,探讨其可能机制。方法利用6-OHDA单侧一点注射大鼠黑质致密区(SNc),特异毁损DA能神经元;术后1、7、14、21 d腹腔注射APO,观察旋转行为;利用免疫组织化学和电镜的方法,观察各时间点黑质DA能神经元形态学变化和纹状体c-fos表达情况。结果毁损侧DA能神经元逐渐减少,超微结构损伤逐渐加重;DA神经元丢失≥80%时,APO诱导的旋转实验>7 r.m in-1,纹状体毁损侧c-fos表达。结论APO能诱导毁损侧纹状体表达c-fos,c-fos表达与DA能神经元毁损程度有一定的关系。 Aim To observe the c-fos expression in the caudate-putman of 6-OHDA-lesioned rats after Apomorphine intraperitoneal injection and to investigate the inherent mechanism of it. Methods Rats received a unilateral injection of 6-OHDA into SNc. The abnormal rotations induced by Apomorphine （APO） were investigated 1,7,14 d and 21 d after lesion. Meanwhile dopaminergic derogation and c-fos expression were observed with Nissl body staining, eletronicmicroscopic and immunocytochemistry methods. Results Thenumber of dopaminergic neurons decreased gradually in the lesioned site and its eletronicmicroscopic structure was damaged. When over 80% dopaminergic neurons were lossed , contralateral rotations surpassed 7 r·min^-1 and c-fos expressed in the ipsilateral striatum. Conclusion APO induces c-fos expression in the ipsilateral striatum. Intrinsic relations may exist between dopaminergic neurons deletion and c-fos expression.

作者冯定庆陈晓蓉

机构地区安徽医科大学组胚教研室

出处《中国药理学通报》 CAS CSCD 北大核心 2005年第9期1119-1122,共4页 Chinese Pharmacological Bulletin

基金安徽省科技厅重点资助项目(No1041184)

关键词 6-羟基多巴胺阿朴吗啡黑质 c—fos 6-hydroxydopamine Apomorphine substantia nigra c-fos

分类号 R-332 [医药卫生] R322.81 [医药卫生—人体解剖和组织胚胎学]

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