自血光量子疗法治疗颈椎病的动物实验

An animal experiment on the treatement of cervical syndrome with autohemo light quanta

目的:观察自血光量子疗法对颈椎病实验动物模型的治疗效果,为临床应用自血光量子疗法治疗颈椎病提供理论依据。方法:实验于2004-03/09在武汉大学人民医院骨科完成。实验动物为健康成年雄性家兔36只,随机分为3组,对照组,颈椎病组和自血光量子组,每组12只。对照组不实施手术造模,颈椎病组及自血光量子组通过手术制备颈椎病实验动物模型。采用自血光量子疗法对自血光量子组动物予以干预,对照组及颈椎病组不予处理。自血光量子组动物按3.5mL/kg体质量自股静脉采血,并与预计采血量1/4的复方枸橼酸钠抗凝液混匀后,置入光量子血疗仪的石英瓶内进行处理。血液处理完毕后自采血静脉快速回输,整个过程严格遵守无菌原则。6个月后,行Rivlin斜板实验(受试家兔分正、反两个方向置于可调节橡胶面斜板上,测定动物在斜板上停留10s的最大倾斜角度,两个方向的角度平均后即为该动物斜板实验的功能角度)观察动物功能恢复。麻醉后处死动物,迅速取出C3～7整段颈椎标本,于冰盐水中剥去硬脊膜和凝血块,经处理后采用可见光分光光度计分别测定血液及颈髓组织中超氧化物歧化酶。结果:所有实验动物均良好存活,全部进入结果分析。①实施手术6个月后,颈椎病组实验动物斜板实验功能角度显著低于对照组及自血光量子组,差异具有显著性。对照组动物同自血光量子组动物接近无差异。②颈椎病组动物血液及颈髓组织中超氧化物歧化酶含量较正常组动物降低[血液:(50.68±9.12)×103,(108.79±8.01)×103NU/L,P<0.05;脊髓:(68.99±7.26)×103,(137.22±10.10)×103NU/L,P<0.05]。经自血光量子疗法治疗后,血液及颈髓组织中超氧化物歧化酶回升至正常水平,与对照组接近[血液(101.07±9.75)×103NU/L,脊髓(124.73±11.21)×103NU/L,P>0.05]。结论:自血光量子疗法可有效提高颈椎病实验动物血液和脊髓超氧化物歧化酶含量、改善机体运动功能,有望应用于临床。

AIM： To observe the therapeutic efficacy of autohemo light quanta therapy in the animal models with cervical syndrome, to provide academic basis for its further clinical application. METHODS： The experiment was done in the Department of Orthopedics, Renmin Hospital, Wuhan University between March and September 2004. A total of 36 adult male rabbits were used and assigned randomly into 3 groups： control group, cervical syndrome group and autohemo light quanta group with 12 rabbits in each group. The rabbits in the control group were not treated with operation to make model, while those in the cervical syndrome group and autohemo light quanta group were treated with the operation to make animal models with cervical syndrome. Intervention was delivered by autohemo light quanta in the autohemo light quanta group. No special treatment was delivered in the other two groups. The blood was gained from self-femoral vein of the animals in the autohemo light quanta group according to 3.5 mL/kg body mass, and after compounding with the sodium citrate anti-coagulation liquor that was prepared 1/4 getting blood volume, they were put into the quartz bottle of light quanta blood therapy meter. After the disposal of blood, the vein blood was inputting, and the whole procedure complied strictly with the sterility principal. Six months later, Rivlin title test （the tested rabbits were laid on the adjustable rubber tihboard in the positive and negative direction, and the maximum tilt angle was measured after staying for 10 s on the tiltboard, when the angle of both direction was average, that angle was the functional angle of the animals in the tiltbeard experiment） was performed to evaluate function recovery of the experimental animal. Then animals were sacrificed after anesthesia, and the whole section of C3-7 cervical vertebra samples was taken rapidly, and the dura mater of spinal cord and coagulum were pilled in the cold salt water. The content of blood and superoxide dismutase （SOD） in cervical spinal tissue was detected by visible light spectrophotometer after disposal.RESULTS： All the experimental animals survived very well, and were all involved in the result analysis.① After 6 months of operation, the functional angle in the cervical syndrome was significantly lower than that in the control group and autohemo light quanta group with significant difference. It was close in the control group and the autohemo light quanta group without difference.② The content of blood and the SOD in cervical spinal tissue in the animals of cervical syndrome group decreased compared with that in the normal group [blood： （50.68±9.12）×10^3, （108.79±8.01）×10^3NU/L, P 〈 0.05; spinal cord： （68.99±7.26）×10^3, （137.22±10.10）×10^3 NU/L, P 〈 0.05]. After the treatment of autohemo light quanta, the content of blood and the SOD in cervical spinal tissue rose up the normal level, and close to that of the control group [blood （101.07±9.75）×10^3NU/L, spinal cord （124.73±11.21）×10^3NU/L, P〉 0.05]. CONCLUSION： The autohemo light quanta therapy can enhance effectively the content of blood and SOD in the experimental animals with cervical syndrome, improve the motor function of body, and is hoped to be used in the clinic.