IL-8和IL-6在中耳胆脂瘤上皮中的表达

Expression of Interleukin-8 and Interleukin-6 in the Middle Ear Cholesteatoma Epithelial

张宏伟陈乾美叶惠平林尚泽梁文妹【摘要】目的探讨白细胞介素-8(IL-8)、白细胞介素-6(IL-6)在中耳胆脂瘤上皮中的表达,分析它们在胆脂瘤上皮骨质破坏中的可能作用。方法应用免疫组织化学SABC法,检测31例患者胆脂瘤上皮和14例患者外耳道皮肤中的IL-8、IL-6的表达情况。结果IL-8主要在31例患者胆脂瘤基底上层细胞和基底层细胞阳性表达,8例患者外耳道皮肤有不同程度表达,两种组织表达的平均吸光度(A值)分别为155.07±13.93、202.15±14.32,有显著性差异(t=10.56,P<0.01)。IL-6主要在30例患者胆脂瘤基底上层细胞和基底层细胞表达,10例患者外耳道皮肤有不同程度表达,两种组织表达的平均吸光度(A值)分别为162.56±20.05、204.15±18.38,有显著性差异(t=6.63,P<0.01)。IL-8与IL-6表达间存在正相关。结论IL-8、IL-6在胆脂瘤上皮高表达,可能与胆脂瘤骨质破坏有关,并可能与IL-1、TNF-α相互作用,共同参与胆脂瘤骨质破坏过程。

Objective To explore the expression of interleukin - 8 （IL- 8）and interleukin - 6 （IL- 6） in the middle ear cholesteatoma epithelium, and to evaluate their roles in the mechanisms of bone destruction. Methods The expression of IL- 8 and IL- 6 was detected by strept avdin - biotin complex （SABC） method. Results All 31 cholesteatoma samples showed stronger expression than the external ear skin. The expression of IL - 8 and IL - 6 could be seen to be strictly confined to the basal and suprabasal cell layer of the cholesteatoma epithelium, while the external ear skin showed weaker expression of IL - 8 and IL - 6. The integral absorbency of IL-8 in these two tissues were 155.07 ± 13.93,202.15 ± 14.32 respectively,with statistically significant difference between them. The integral absorbency of IL- 6 in the two tissue type were 162.56± 20.05,204.15 ± 18.38 respectively, with statistically significant difference between them. There was correlation between the expression of IL - 8 and IL - 6. Conclusion The overexpression of IL - 8 and IL - 6 in the middle ear cholesteatoma epithelium may play an active role in the mechanism of cholesteatoma epithelium invasion into the bone. IL-8 and IL-6 may interact on IL- 1 and TNF-α,which may be involved in the bone destruction of cholesteatoma epithelium.