体外培养的食管癌细胞光动力疗法的电镜观察

Electronic Microscopic Obser vation on the Effect of Photodynamic Therapy on Esophageal Carcinoma Cells

背景与目的:研究光动力疗法对食管癌细胞超微结构的影响。材料与方法:培养的食管癌细胞分别与光敏剂作用6、12和24h后,再经紫外线(UltravioletB,UVB)照射0.5h。通过透射电镜观察各实验组和对照组食管癌细胞的超微结构。结果:电镜下可见实验组食管癌细胞出现线粒体固缩,粗面内质网减少,滑面内质网扩张,核膜断裂,核仁固缩。上述膜性结构的损伤随着光敏剂作用时间延长而加重。结论:光动力疗法可导致食管癌细胞超微结构,尤其是膜性结构损伤,因而可能具有治疗食管肿瘤的效应。

BACKGROUND ＆ AIM： To study the effect of photodynamic therapy （PDT）on ultrastructure of esophageal carcinoma cells, MATERIAL AND METHODS： After action of photosensitizer for 6, 12, and 24 hours respectively, cultured esophageal carcinoma cells were irradiated with Ultraviolet B（UVB） for half an hour. The ultrastructure of esophageal carcinoma cells in experimental groups and control groups were observed under transmission electron microscope. RESULTS： PDT resulted in condensation of mitochondria, reduction of rough endoplasmic reticulum, dilation of smooth endoplasmic reticulum, breakup of nuclear membrane and pyknosis of nucleolus. The severity of injury of membranous structures increased with the time prolonging of photosensitizer action. CONCLUSION： PDT can do damage to membranous structures of esophageal carcinoma cells, which suggests that PDT may have clinical efficacy for treatment of esophageal carcinoma.