P21^(WAF1/CIP1)、P16蛋白表达与人脑神经胶质瘤的相关分析

Correlation Analysis Between Expression of P21 ^(WAF1/CIP1) ,P16 Proteins and Human Gliomas

背景与目的:探讨P21WAF1/CIP1、P16两种抑癌基因与人脑神经胶质瘤恶性程度的关系。材料与方法:采用SABC免疫组织化学方法对98例人脑胶质瘤组织及12例正常脑组织标本中P21WAF1/CIP1和P16的表达进行检测,并进行相关分析。结果:P21WAF1/CIP1和P16阳性表达率在人脑胶质瘤中分别为58.16%和42.86%,与正常脑组织中的表达情况差异有统计学意义;P21WAF1/CIP1蛋白和P16蛋白阳性表达率均随着胶质瘤的恶性程度的增高而降低;P21WAF1/CIP1蛋白和P16蛋白可协同表达。结论:P21WAF1/CIP1与P16蛋白的阳性表达率及协同表达率可在一定程度上反映胶质瘤细胞的恶性生物学行为,可以作为一项判断其恶性程度的有效指标。

BACKROUND ＆ ATM： To investigate the relationship between glioma malignancy grading and P21^WAF1/CIP1、P16 gene expressing levels. MATERIAL AND METHODS： The expression levels of P21^WAF1/CIP1 and P16 proteins were detected in 98 cases of brain glioma using SABC immunohistoehemical staining. Eight normal brain tissues were used as control. RESULTS： The positive expression rates of P21^WAF1/CIP1 and P16 protein in glioma were 58.16% and 42.86% respectively, anda significant difference was observed between gliomas and control. The expression intensity of P21^WAF1/CIP1 and P16 decreased with the gliomas grade increasing. Coexpression of P21^WAF1/CIP1and P16 was found in glioma and normal brain tissues. CONCLUSION： The positive expression rate and coexpression rate of P21^WAF1/CIP1 and P16 protein may reflex the malignant grade of glioma in some extent, and they can be as a sensitive index for the gliomas grading.