HPLC法测定阿维菌素缓释注射液中阿维菌素B_1的含量

Determination of avermectin B_1 in avermectin sustained-release injection by HPLC

目的:建立阿维菌素(AVM)缓释注射液中阿维菌素B1(AVM B1)的高效液相含量测定方法.方法:采用HPLC方法测定自制的AVM缓释注射液中AVM B1的含量.色谱柱:Hypersil-C18(200 mm×4.6 mm,5 μm),流动相为甲醇-水(85∶15),流速1 ml/min,检测波长245 nm.结果:AVM B1在0.695 8～6.957 5 μg(r=0.999 2)范围内线性关系良好,平均回收率为99.10%(RSD＝0.89%),日内及日间精密度RSD分别为0.59%、0.67%.结论: HPLC法操作简单、快速、准确,可用于阿维菌素缓释注射液中AVM B1含量的测定.

Objective： To establish a HPLC method for the determination of Avermectin B1 in Avermectin sustained-release injection which was made by ourself. Methods： SHIMADZU VP-ODS（200 mm×4. 6 mm, 5μm） column was used. The mobile phase consisted of methanol-water（85：15,v/v）. The flow rate was 1 ml/min. Detection wavelengths was set at 245 nm. Results：The linear range of AVM B1 was 0. 6958-6. 957 5μg （r=0. 999 2）. The average recovery was 99.10%（RSD=0.89%）. Conclusion： This method is simple, rapid and accurate, and can be used for the determination of Avermectin B1 in Avermectin sustained-release injection.