鸭源H10亚型禽流感病毒血凝素基因的序列分析及其致病特性

Sequence analysis of hemagglutinin gene of duck origin avian influenza virus H10 subtype and pathogenicity to SPF chickens and BALB/c mice

采用血清学试验和RT-PCR方法从华东地区家养水禽中分离鉴定出多株H10亚型禽流感病毒,对其中1株A/Duck/Yangzhou/502/2003(简称Dk/YZ/502/03)的血凝素(HA)基因进行了序列测定,并与GenBank中登录的其他序列进行了比较。结果显示,Dk/YZ/502/03株血凝素基因与哺乳动物水貂分离株A/Mink/Sweden/84(H10N4)的同源性最高;其推导的氨基酸剪切位点序列为P-E-I-M-Q-G-R,为典型低致病性禽流感病毒的特征序列,这与该毒株对SPF鸡和BALB/c小鼠的低致病特性相吻合。

Several H10 subtype avian influenza A viruses were isolated from aquatic birds in East China and identified by the specific RT-PCR. The hemagglutinin （HA） gene of one representative virus strain Dk/YZ/502/03 out of the viruses was sequenced and pathogenicity of the strain to SPF chickens and BALB/c mice was determined. The results were as follows. The HA gene＇s nucleotide sequence of the strainDk/YZ/502/03 shared high identity with that of A/Mink/Sweden/84（H10N4）, and the sequence P-E-I-M-Q-G-R of cleavage site of the anino acid deduced from the gene was the characteristic sequence of avian influenza viruses with low pathogenicity, which agreed with the low pathogenicity of the strain Dk/ YZ/502/03 to SPF chickens and BALB/c mice.