摘要
为了进一步提高抗A、抗B血型单克隆抗体试剂的稳定性,实验中分别以不同配比的甘油、蔗糖、明胶和EDTA为稳定剂主要成分加入血型单抗试剂,放置在不同条件下观察试剂的稳定性.结果表明,所筛选出的一种稳定剂对血型单抗试剂具有良好的保护作用,试剂在2～8℃条件下放置12个月,凝集效价保持不变,并且该稳定剂对血型单抗的特异性、亲和力、凝集效价和凝集强度均无影响.
To improve the stability of anti-A and anti-B blood grouping reagents （ monoclonal antibody）, glycerin, sucrose, gelatin and EDTA were used as components of stabilizers in the designed formulations with different proportion. The results showed that the selected formulation had a significant protective effect on blood grouping reagents, and the agglutination titern of reagents were kept immovability at 2 - 8℃ condition for 12 months. The results also showed that the selected stabilizers had no effect on reagent＇s specificity, affinity, agglutination titer and agglutination intensity.
出处
《微生物学免疫学进展》
2005年第3期37-40,共4页
Progress In Microbiology and Immunology
关键词
血型试剂
单克隆抗体
稳定剂
Blood grouping reagents
Monoclonal antibody
Stabilizer
