产AmpC酶阴沟肠杆菌的基因分析及其耐药性

Analysis gene of strains of enterobacter cloacae producing AmpC β-lactamase and its resistance

探讨昆明地区阴沟肠杆菌的耐药性及与结构基因ampC和调节基因ampD的相关性。通过K-B法检测阴沟肠杆菌的药敏情况,头孢西丁三维试验检测AmpC酶,PCR法扩增ampC和ampD基因。结果显示74株阴沟肠杆菌经头孢西丁三维试验检测,产AmpC酶的有17株,检出率为22.3%,而且产酶菌株抗生素敏感率低于非产酶菌株。ampC基因扩增阳性率为89.2%(66/74);64株ampD基因阳性率为86.5%(64/74)。实验证实昆明地区产酶阴沟肠杆菌耐药状况严重,与结构基因ampC和调节基因ampD密切相关。

To investigate the resistance among clinical isolates of Enterobacter cloacae from Kunming and the relationship between drug resistance and AmpC structure gene and AmpD regulative gene. K-B tests were performed to detect the susceptihility of antimicrobial agents, AmpC β-lactamase in Enterobacter cloacae was detected by three-dimenslonal test. AmpC and AmpD genes were detected by PCR. AmpC β-lactamase was found 22.3% in 74 strains of Enterobacter cloacae by three-dimensional test. The sensitive rates of drug to the strains producing AmpC β-lactamase were lower than those of strains not producing AmpC β-laetamase. AmpC gene was positive in 66 of 74 tested isolates （ 89.2% ） and AmpD gene was positive in 64 of 74 tested isolates （ 86.5% ） by three-dimensional test. The resistant status of Enterobacter cloacae from Kunming is in a severe situation. The structure gene of AmpC and regulative gene of AmpD of Enterobacter cloacae may be associated with the drug resistance.