芦荟属植物种间杂交及其F_1代POD同工酶鉴定

The interspecific hybridization and identification of F_1 hybrids by POD isozyme application in Aloe

以芦荟属植物元江芦荟和皂质芦荟为亲本进行远缘有性杂交试验。正交对3株103朵小花进行授粉,获得7枚果实,平均结实率为6.80%;反交对2株88朵小花授粉,获得5枚果实,平均结实率为5.88%。获得了19株F1代实生苗,并对亲本和F1代实生苗以及库拉索芦荟、华芦荟等5种芦荟属植物进行了过氧化物酶同工酶(POD)的比较研究,结果显示,5种芦荟种间的POD同工酶酶谱具有较高的相似程度,同时各种又具有各自的特征酶带,很容易区分。特别是F1代实生苗的酶谱与父母本的酶谱有显著不同的酶带,证明产生了新的中间类型。

In the paper,we described a distant sexual hybridization experiment in which 19 F1 seedlings were generated from maternal plant Aloe yuanjiangensis Xiong, Zheng et Liu of Aloe L. and paternal plant A. saponaria（Ait. ）Haw. 7 fruits were obtained from direct cross, average fructicative rate was 6.80%;5 fruits were obtained from reciprocal cross, average rate was 5. 88%. By the comparing research of peroxide isoenzyme（ POD） on the 5 species of plants of Aloe L. ：the paternal and maternal plants, F1 seedlings,A, vera L. ,A. chinensis（Haw. ）Baker,etc. It is easy to distinguish the respective characteristic enzyme zones of the 5 species of plants of Aloe L. although their enzyme spectrums are rather similar. Particularly, the distinctive differences between F1 seedlings and their paternal and maternal plants proved that the new intermediate types were generated.