三氧化二砷对小鼠过敏性哮喘的治疗作用观察

The Therapeutic Efficacy of Arsenic Trioxide in Asthma in Mice

目的:观察三氧化二砷对小鼠过敏性哮喘的治疗作用。方法:49只雄性小白鼠随机分为7组:对照组,哮喘模型组,哮喘+地塞米松2mg/kg治疗组,哮喘+三氧化砷0.575mg/kg,哮喘+三氧化二坤1.15mg/kg治疗组,哮喘+三氧化砷2.3mg/kg治疗组,哮喘+三氧化二砷4.6mg/kg治疗组。每组各7只。用卵蛋白建立小鼠过敏性哮喘模型,收集肺泡灌洗液(BALF),直接计算白细胞总数和分类,双缩脲法检测总蛋白含量,应用Medlab生物信号采集系统软件检测小鼠肺功能。结果:三氧化二砷治疗组BALF中总蛋白含量,白细胞总数,巨嗜细胞,嗜酸细胞和淋巴细胞与哮喘组比较,有显著性差异(P<0.05,P<0.01);三氧化二砷治疗组肺功能中Pmax和T呼/T总与哮喘组比较,有显著性差异(P<0.05,P<0.01)。结论:三氧化二砷对小鼠过敏性哮喘有治疗作用。

Objective：To study the therapeutic efficacy of arsenic trioxide in asthma. Methods：Forty --nine healthy kunming male mice at 5 weeks of age were randomly divided into seven groups： control group, asthmatic group, dexamethasone group and arsenic groups （the mice were given arsenic trioxide 0. 575 mg/kg,1.15 mg/kg ,2.3 mg/kg,4.6 mg/kg before challenge）. An animal model of asthma was established by means of OVA sensitizing-challenging. Pulmonary function in those mice was measured by Organism Signal Collectio leucocytes, eosinophils, lymphocytes and protein concentrations decrease. Results：In those groups treated with arsenic trioxide, compared with astbmatic group, total protein concentration, total leucocytes, eosinophils and lymphocytes were decreased（P〈0. 05, P〈0.01）, Pmax and Tbreath/Ttotal of the pulmonary function was improved（P〈0.05, P〈0. 01）. Conclusion： There are therapeutic effects of arsenic trioxide on asthma, the count of total.