克林霉素诱导型耐药葡萄球菌的检测与分析

Detection for inducible clindamycin-resistant of staphylococcus isolates and analysis of resistance

目的了解本地区葡萄球菌诱导型克林霉素的耐药率。方法用K-B琼脂扩散法检测葡萄球菌对红霉素和克林霉素的耐药性,用D-试验检测克林霉素诱导型耐药。结果61株耐红霉素的金黄色葡萄球菌对克林霉素耐药40株,敏感21株,其中克林霉素诱导型耐药8株;106株耐红霉素的凝固酶阴性的葡萄球菌(CNS)对克林霉素耐药62株,敏感44株,其中克林霉素诱导型耐药27株。结论对克林霉素敏感的菌株,应加做D-试验以免造成错误判断导致临床治疗失败。

Objective To investigate the frequency of inducible clindamycin-resistant of staphylococcus isolates in the local district. The susceptibilities of erythromycin and clindamycin for staphylococcus were examined by Kirby-Bauer disc agar diffusion test and the inducible clindamycin-resistance was detected by D-test. Results Of 61 erythromycin-resistant Staphylococcus aureu isolates, 40 were demonstrated to be resistant to clindamycin,while 21 showed susceptible to clindamycin, among them 8 were inducible clindamycin-resistant isolates. Of 106 erythromycin-resistant coagulase-negative staphylococcus（CNS） isolates,62 were demonstrated to be resistant to chndamycin, while 44 showed susceptible to clindamycin, among them 27 were inducible resistant isolates. Conclusion The percentage of inducible clindamycin-resistance was 53.8% （35/65） in clindamycin-susceptible staphylococcus isolates. The results indicated that D-test should be performed in clindamycin-susceptible isolates which were failing in clinical therapy by using clindamycin for staphylococcal infection.