ADVIA120血液分析仪过氧化物酶鞘液试剂的研制与应用

Preparation and application of self-made peroxidase sheath reagent for ADVIA 120 hemocyte analyzer

目的探讨自配过氧化物酶鞘液试剂是否能应用于ADVIA120血液分析仪。方法分析原装试剂成分、理化指标;研制效果相同的试剂,并作一系列比对试验。结果自配试剂的各项检测指标:N(%)、L(%)、M(%)、E(%)、B(%)、LUC(%)、WBCP等与进口试剂的检测结果基本一致,无显著性差异。结论自配的过氧化物酶鞘液试剂可替代进口试剂用于ADVIA120血液分析仪。

Objective To investigate if the self-made peroxidase sheath reagent can be used to substitute for PEROX SHEATH in ADVIA120 hemocyte analyzer. Methods The components and the physical and chemical parameters of original reagent of ADVIA 120 hemocyte analyzer were analyzed, and then the similar reagent was prepared. A series of comparisons between the self-made and original reagents were observed. Results All the parameters, e. g. , Neut （ % ）, Lym （ % ）, Mono （ % ）, Eos （ % ）, Baso （ % ）, LUC （ % ） and WBCP,were analyzed by using the self-made reagent, and the results were similar to those analyzed by the original reagent. No significant difference between the two reagents was found and satisfactory results were achieved during three-month clinical trial. Conclusions The self-made peroxidase sheath reagent can be used to replace the imported original reagent PEROX for ADVIA 120 hemocyte analyzer.