海马酶法提取物的抗疲劳作用

Anti-fatigue effect of enzymatic extract of hippocampus

为了解海马酶法提取物的抗疲劳作用,制备一次性运动疲劳模型小鼠和递增运动量疲劳模型小鼠,(后者的制备方法是:小鼠给予体重3%的负荷进行游泳,每天定时1次连续4d,第1天游泳时间30min,然后每天递增10min)。测定上述2种疲劳小鼠的血乳酸、血清尿素氮和后肢骨骼肌脂质过氧化程度。结果表明,海马酶解提取物(Enzymaticextractsofhippocampus,EEH)即可减少运动时乳酸、尿素氮和过氧化物的产生,又能促进运动后3者的清除;EEH的作用与机体的运动状态有关,随着运动量的增加和疲劳程度的加重,EEH的作用也增强。此外,对于正常小鼠,EEH可延长其在缺氧、寒冷以及缺氧合并寒冷条件下的生存时间,延长游泳时间。EEH的上述作用均呈明显的量效关系。在本试验条件下EEH具有明显的抗疲劳作用。

Hippocampus has long been used as an ingredient in Chinese traditional medical prescriptions. To explore the health protection effect of hippocampus, four groups of NIH mice were given enzymatic extract of hippocampus （EEH） 0, 50, 100, 200 mg/kg BW respectively by gavage daily for 14 days. On the 14th day, 1 hour after the last dose was given, the animals＇ capacity to tolerate fatigue was tested by physical exercise （swimming） of various intensity. The level of blood lactic acid, serum urea nitrogen and lipid-peroxidation of skeletal muscle of hind leg were used as indexes of the capacity of tolerance. The results showed that the rise of these indexes was significantly lower and their clearance was faster after swimming in animals received EEH than those in the animals of the control group. It was also observed that the anti-fatigue action of EEH was dose-dependent, the larger the dose the stronger the action. Furthermore, EEH＇s effect was also related to the physical condition of the animal. The anti-fatigue effect enhanced with the increase of the intensity of physical exercise. In an additional test, EEH was found to have the effect of withstanding hypoxia and coldness.