胶体金免疫层析法快速检测氯霉素残留

Nanocolloidal gold-based immunoassay for rapid detection of chloramphenicol residues

为建立用于快速检测样品中氯霉素残留含量的胶体金免疫层析试纸条,采用免疫竞争法,将抗氯霉素单克隆抗体-胶体金复合物包被在胶体金结合垫上,并将人工合成的氯霉素抗原包被在硝酸纤维素薄膜表面作为检测线(T线),其与待测样品中氯霉素竞争结合胶体金标记的氯霉素单克隆抗体,并能以颜色直观显示检测的定性结果.检测虾肉等组织试样时,灵敏度最低值可达到1 ng/ml,只需5～10 min,与类似物无交叉反应.试纸条具有较高的灵敏度及特异性,操作便捷,稳定可靠,可作为氯霉素残留现场监控的有效筛检手段.

A rapid, simple colloidal gold immunochromatographic assay for detecting chloramphenicol （CAP） residues has been established. Nanocolloidal gold particles were prepared and labeled to an anti-CAP monoclonal antibody （MAb）. CAP was conjugated to bovine serum albumin （BSA） and dispersed on a nitrocellulose （NC） membrane to be the test line （T）. The more analyte present in the sample, the more effectively it would compete with the CAP- BSA for binding to the limited amount of gold-labeled anti-CAP MAb. The presence or absence of a colored band on the test line indicates a negative or positive result. When measuring the water sample spiked with chloramphenicol, the minimum detection concentration can reach 1 ng/ml. The major advantage of the one-step strip test is that the test can be completed in less than 10 minutes.