摘要
为建立高效液相色谱-紫外-电喷雾质谱(HPLC-UV-ESI/MS)联用测定保健食品中荷叶碱的方法,以乙腈和0.1%三乙胺水溶液作流动相,在反相C18柱上梯度淋洗,以质谱定性,紫外定量进行检测.结果在0.2～20 μg/ml范围内峰面积和浓度呈良好的线性关系,样品平均回收率为97.3%.该方法抗干扰能力强,可以快速、准确地测定保健食品中的荷叶碱.
A method for determining nuciferine in functional foods by on line HPLC - UV - ESI/MS was established. The separation was operated on an analytical Spherigel C18 column （5μm, 250 mm × 4.6 mm） with acetonitrile： water （containing 0.1% triethylamine） as mobile phase. The detection wavelength was 280 nm. Good linearity between peak areas and concentrations was obtained within the range of 0.2 μg/ml - 20μg/ml. The method is simple and can determine nuciferine in functional foods accurately.
出处
《中国食品卫生杂志》
2005年第5期420-421,共2页
Chinese Journal of Food Hygiene
基金
国家"十五"重大科技专项(2001BA804A21)
新药博士基金(2003AA2Z3515)
湖南省杰出青年科学基金(03JJY1002)~~
关键词
莲
生物碱
色谱法
高压液相
光谱分析
质量
营养保健品
NELUMBO NUCIFERA
ALKALOIDS
Chromatography, High Pressure Liquid
Spectrum Analysis, Mass
Dietary Supplement
