ALDH2基因对酒精性心肌病影响的评价

Relationship between genic polymorphism of ALDH2 and alcoholic cardiomyopathy

目的研究ALDH2基因多态性与酒精性心肌病的关系。方法采用前瞻性研究,随机选择56例正常人和64例嗜酒的非酒精性心肌病及非酒精性肝病患者及82例酒精性心肌病患者为研究对象,应用聚合酶链反应限制性片段长度多态性方法检测ALDH2基因多态性。结果嗜酒组和酒精性心肌病组ALDH2!1/!1型频率高于对照组(P<0.01),ALDH2!1/!2及ALDH2!2/!2型频率低于对照组(P<0.01)。嗜酒组和酒精性心肌病组ALDH2!1等位基因的频率高于对照组(P<0.01),ALDH2!2等位基因的频率低于对照组(P<0.01)。嗜酒组和酒精性心肌病组各种基因型频率及等位基因的频率无统计学差异。结论ALDH2基因多态性与嗜酒相关,ALDH2!1等位基因在嗜酒的形成中起一定作用;ALDH2基因多态性与酒精性心肌病发生的关系未得到证实。

[Objective] To reveal the associations of the Polymorphism of ALDH2 with alcohol dependence and alcoholic eardiomyopathy. [Methods] In this study, ALDH2 polymorphism were examined in 56 controls and 56 patients with alcohol dependence and 82 patients with alcoholic eardiomyopathy. Polymerase chain reaction-restricted fragments length polymorphism was used to determine genotypes of ALDH2. [Results] The genotype frequency of ALDH2 ＊ 1/＊ 1 between alcoholic eardiomyopathy group and alcohol dependent group was significantly higher than that in controls. The genotype frequency of ALDH2 ＊ 1/＊ 2 and ALDH2 ＊ 2/＊ 2 between alcoholic eardiomyopathy group and alcohol dependent group was significantly lower than that in controls. The allele frequency of ALDH2 ＊ 1 between alcoholic eardiomyopathy group and alcohol dependent group was significantly higher than that in controls and the allele frequency of ALDH2 ＊ 2 between alcoholic eardiomyopathy group and alcohol dependent group was significantly lower than that in controls. The genotype and allele frequency of ALDH2 in alcoholic eardiomyopathy group and alcohol dependent group were no dlfferenee. [Conclusion] The allele of ALDH2 ＊ 1 may play a role in alcohol dependent patient. The relationship of the Polymorphism of ALDH2 with aleohohe eardiomyopathy is not confirmed.