普洱茶遗传毒性安全性评价研究

Genetic Toxicity Evaluation of Pu'er Tea

以烘青绿茶作对照,用经典方法对云南产代表性厂家生产的一年、五年和十年三个普洱茶产品进行了遗传毒性安全性评价研究。四个茶样的小白鼠骨髓微核试验、畸形精子检测试验和Ames试验结果均为阴性,表明所测试的普洱茶样和烘青绿茶样对原核细胞、真核细胞和生殖细胞都没有致突变性。排除了普洱茶对引起染色体断裂损伤、基因碱基置换和移码等致突变性。试验还发现试样对其他因素引起的微核率的增加有拮抗作用。从遗传毒性证明了四个茶样具有较高的食用安全性,表明正规厂家生产的普洱茶是安全的。

The investigation on the safely evaluation based on the genetic toxicity was conducted on those three representative on Puer tea sample with one year, five years and ten years storage period produced by the factory of Yunnan by using the classical testing method and used the roasted green tea as the control. The results of mice bone marrow micronuclei test, the mice abnormal spermatozoon test and the Ames test of four tea samples showed negative reaction, Including no mutagenicity on primitive cells, authentic cells and reproductive cells by tested Pu＇er tea and roasted green tea samples. The experiment excludes the mutagenicty including the chromosome breakage damage, gene base-pair substitution, and frame shift induced by Pu＇er tea. Investigation also discovered that the test samples showed antagonic on the increase of micronucleus rate induced by other factors. Results showed that there four tea samples showed a higher safety to human cell and the Pu＇er teas produced by the regular factory are safe to consumer.