疏肝理肺方防治应激条件下大鼠哮喘的作用机制研究

Protective and therapeutic effects of Shugan Lifei Recipe on rats with asthma under stress and the mechanisms

目的:探讨心理因素对哮喘发病的影响和调肝法调节应激、防治哮喘的作用机制。方法:用卵蛋白加束缚应激刺激建立应激条件下哮喘大鼠模型,采用放射免疫分析法、酶联免疫吸附法、原位杂交、免疫组化、光镜及电镜等方法,检测各组大鼠血浆皮质酮、白细胞介素4、干扰素γ、海马糖皮质激素受体mRNA和下丘脑促肾上腺皮质激素释放激素(adrenocorticotropichormonecorticoliberin,CRH)阳性神经元,并观察肺和海马组织形态学的变化。结果:疏肝理肺方可明显降低血浆皮质酮水平,升高血浆干扰素γ,降低血浆白细胞介素4,减少下丘脑室旁核CRH阳性神经元,上调海马CA3区糖皮质激素受体mRNA的表达,减轻海马神经元的损伤,并可明显减轻应激条件下哮喘大鼠肺组织的病理改变。结论:疏肝理肺方通过抑制应激条件下哮喘大鼠下丘脑-垂体-肾上腺皮质轴功能的亢进及调节免疫系统的功能,从而起到防治哮喘的作用。

Objective： To explore the influence of psychological factor on asthma, the protective and therapeutic effects of Shugan Lifei Recipe on rats with asthma under stress and the mechanisms. Methods： Allergy and stress-induced asthma models were established in rats by giving ovalbumin and restraining stress. Radioimmunoassay, enzyme linked immunosorbent assay, in situ hybridization and immunohistrochemical method were used to detect plasma corticosterone, IL-4, IFN-γ, expression of glucocorticoid receptor （GR） mRNA in hippocampus and adrenocorticotropic hormone corticoliberin （CRH） positve neuron in hypothalamus. Optical and electron microscopes were used to observe the morphological changes of pulmonary hilar and hippocampal tissues. Results： Shugan Lifei Recipe （SGLFR） could reduce plasma corticosterone, decrease CRH positive neurons in paraventricular nucleus of hypothalamus, up-regulate the expression of GR mRNA in hippocampus and reduce the injury of hippocampal neuron. SGLFR had certain inhibitive effect on hyperfunction of hypothalamo-pituitary-adrenal cortex （HPA） axis in rats with asthma under stress and could also relieve the pathological changes of the pulmonary hilar tissue. The level of plasma IFN-γ was increased while the level of plasma IL-4 was decreased in SGLFR-treated group. Conclusion： The mechanism of SGLFR in treating the rats with asthma under stress is probably to regulate the hyperfunction of HPA axis and the disorder of immuo-system.