摘要
目的:观察益气、活血方药对老年大鼠肝脏衰老相关基因表达的影响。方法:以自然衰老大鼠为衰老模型,采用RT-PCR、Westernblotting法研究益气、活血方药对老年大鼠肝脏p16、p21、增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)、cyclinD1和cyclinEmRNA转录和蛋白表达的影响。结果:老年大鼠肝脏p16、p21、cyclinD1mRNA转录和蛋白的表达增加;PCNA、cyclinEmRNA转录和蛋白的表达降低。与老年大鼠比较,益气、活血方药可下调老年大鼠肝脏cyclinD1mRNA转录和蛋白表达,上调促进增殖基因cyclinEmRNA转录和蛋白表达,但对p16、p21和PCNA的调节作用不明显。结论:益气、活血方药主要通过调控eyclinD1和cyclinEmRNA转录和蛋白表达而促进细胞增殖。
Objective： To observe the effects of recipes for replenishing qi and activating blood on p16, p21, proliferating cell nuclear antigen （PCNA）, cyclin D1 and cyclin E gene expressions in the liver of aging rats. Methods： A recipe for replenishing qi and a recipe for activating blood were administered to aging rats respectively, and the effects of the above recipes on the expressions of senescence related genes （p16, p21, PCNA, cyclin D1 and cyclin E） were examined by RT-PCR and Western blotting methods. Results： The expressions of p16, p21 and cyclin D1 mRNAs and proteins in the liver of the untreated aging rats were upregulated, while the expressions of PCNA and cyclin E mRNAs and proteins decreased. As compared with the untreated aging rats, both recipes could down-regulate the expressions of cyclin D1 mRNA and protein and upregulate the expressions of cyclin E mRNA and protein, but had no obvious effects on the expressions of mRNAs and proteins of p16, p21 and PCNA. Conclusion- Recipes for replenishing qi and activating blood can improve the liver cell proliferation of aging rats via down-regulating the expressions of cyclin D1 mRNA and protein and up-regulating the expressions of cyclin E mRNA and protein.
出处
《中西医结合学报》
CAS
2005年第5期370-373,共4页
Journal of Chinese Integrative Medicine
基金
国家自然科学基金资助项目(No.30070914)
