中药地椒乙醇提取物对人白血病细胞增殖的抑制作用

Anti-tumor effect of ethanol extracts from Thymus quinquecostatus Celak on human leukemia cell line

目的:筛选中药地椒乙醇提取物的抗肿瘤活性成分,探讨其可能的抗肿瘤作用机制。方法:从野生地椒乙醇提取物中分离得到乙酸乙酯、正丁醇和丙酮3种萃取组分,以体外培养的人白血病细胞株K562和HL-60作为研究对象。将各萃取组分与肿瘤细胞作用一定时间后,计数存活肿瘤细胞数,分析药物对肿瘤细胞生长曲线的影响,考察各组分对肿瘤细胞生长的抑制作用。以去甲斑蝥素作为阳性对照组,通过形态学分析等方法,研究中药地椒乙醇提取物可能的抗肿瘤作用机制。结果:中药地椒乙酸乙酯萃取物对人白血病细胞株K562和HL-60均有显著的抑制作用,且呈药物浓度依赖性,并可诱导肿瘤细胞凋亡;而正丁醇和丙酮萃取物对两种白血病细胞均无显著的抑制作用。结论:体外实验表明,乙酸乙酯萃取物是中药地椒乙醇提取物中主要的抗肿瘤活性成分。

Objective： To screen the anti-tumor fraction of ethanol extracts from Thymus quinquecostatus Celak and investigate its anti-tumor effect on human leukemia cell line, Methods： Ethyl acetate, n-butanol and acetone fractions were separated from the ethanol extracts of wild Thymus quinquecostatus Celak, Growth inhibiting effects of these extracts on human leukemia cell lines K562 and HL-60 were determined by live cell counting and cell growth curve analysis. The possible anti-tumor mechanism was studied by morpho- logical analysis with norcantharidin as a positive control. Results： Ethyl acetate fraction could significantly inhibit the proliferations of K562 and HL-60 cells, and the inhibiting effect depended on the concentration of ethyl acetate fraction. Ethyl acetate fraction could induce apoptosis of K562 and HL-60 cells. The n-butanol and acetone fractions had no significant inhibiting effect on K562 and HL-60 cells, Conclusion： Ethyl acetate fraction is the major anti-tumor fraction in ethanol extracts from Thymus quinquecostatus Celak.