低聚木糖生产废渣纤维素酶诱导和水解特性的研究

CHARACTERISTICS OF CELLULASE INDUCEMENT AND HYDROLYSIS BY RESIDUE OF XYLOOLIGOSACCHARIDE PRODUCTION

研究了生产低聚木糖(XOS)所得的废渣对里氏木霉纤维素酶合成的诱导作用和纤维素酶水解特性。废渣对里氏木霉合成纤维素酶的诱导作用较差,而纤维素酶水解性能优异。里氏木霉以含纤维素15g/L的废渣为碳源合成纤维素酶,滤纸酶活为0.48FPIU/mL,酶产率为6.67FPIU/(L·h),酶得率为每克纤维素32.00FPIU,而在相同条件下以玉米芯为碳源时滤纸酶活为3.20FPIU/mL、酶产率19.00FPIU/(L·h)和酶得率每克纤维素213.33FPIU。质量浓度为20g/L的废渣在酶用量为每克纤维素10FPIU条件下水解24h,水解得率达92.8%;底物废渣质量浓度为100g/L时,48h纤维素酶水解得率达到80.6%。

The characteristics of cellulase inducement and hydrolysis by residue of xylooligosaccharide （XOS） production were investigated. The residue of XOS production was sensitive to cellulase, but it was a poor inducer for cellulase biosynthesis. The filter paper activity, volumetric productivity and yield by Trichoderrna reesei, which employed residue of XOS production containing cellulose 15 g/L as carbon source, were 0.48 FPIU/mL, 6.67 FPIU/（L · h） and 32 FPIU/g cellulose, respectively. Compared to those of 3. 20 FPIU/mL, 19. 00 FPIU/ （L · h） and 213.33 FPIU/g cellulose, which used untreated corncobs as carbon source. The enzymatic hydrolysis yield was 92.8 %when 20 g/L residue of XOS production was treated with the dosage of 10 FPIU/g cellulases for 24 h, and when the substrate concentration was improved to 100 g/L,the hydrolysis yield was 80.6 % for 48 h under the same conditions.