四季青中一个新的酚性化合物

A NOVEL PHENOLIC COMPOUND FROM LEAVES OF ILEX PURPUREA HASSK.

对冬青科植物冬青干燥叶(四季青)的化学成分进行了研究。取干药材10kg,加8倍量体积分数80%乙醇回流煎煮3次,每次4h,回收乙醇至无醇味。浸膏混悬于适量水中,依次用石油醚、乙酸乙酯、正丁醇萃取,回收溶剂。采用不同层析技术进行分离,从正丁醇部位分得1个酚性化合物,经理化及光谱方法鉴定为:3,4-二羟基-7-(3′-O-β-D-吡喃葡萄糖基-4′-羟基-苯甲酰基)-苯甲醇(俗称四季青酚苷),一种新化合物。

To study the constituents in leaves of llex purpreae Hassk. , which are used as one kind of traditional Chinese medicine, 10 kg dried leaves were extracted with 80 % EtOH （8 times of the materials ） for 3 times, each time for 4 h. The filtrate was concentrated in vaccum to remove ethanol completely, The residue was suspended in water,extracted successively with petroleum ether, ethyl acetate and n-BuOH. The n-BuOH fraction was subjected to different column chromatography for isolation and purification. A phenolic compound was isolated. Its structure, elucidated by chemical and spectral methods, was 3,4-dihydroxy-7- （ 3 ＇-O-β-D-glucopyranosyl- 4＇-hydroxy-benzoyl ） - benzylalcohol, which is a novel natural product.