摘要
采用超临界CO2萃取(SCDE)技术,研究了从箬竹叶中提取叶绿素的工艺。对影响SCDE叶绿素的各种因素,包括萃取温度、萃取压力、萃取次数及夹带剂选择等进行了单因素试验研究,得到较适宜的萃取工艺条件为:萃取温度50℃、萃取压力25MPa、夹带剂用95%(体积分数)乙醇(其用量为每克原料8mL)、萃取次数4次。在上述较适宜的萃取工艺条件下,得到总叶绿素得率0.45%。本试验为研究从箬竹叶中萃取分离叶绿素提供了一种新的工艺方法。
The extraction and isolation conditions herklotsii McClure with supercritical CO2 extraction of chlorophyll were studied using the leaves of Indocalamus （SCDE）. Influences of extraction temperature, pressure, and time on the content and purity of chlorophyll were investigated, and suitable conditions were determined based on single-factor tests as follows： extraction temperature 50 ℃, extraction pressure 25 MPa, 95 % alcohol （volume part） as a modifier was used at dosage of 8 mL per gram material, extraction for 4 times. The yield of chlorophyll was 0.45 % under the above conditions. A new method for isolating chlorophyll from leaves of I. herklotsii was provided in this research.
出处
《林产化学与工业》
EI
CAS
CSCD
2005年第3期81-84,共4页
Chemistry and Industry of Forest Products
基金
江苏省高校自然科学研究指导性计划资助项目(03KJD220351)
关键词
超临界CO2萃取
叶绿素
箬竹叶
supercritical carbon dioxide extraction
chlorophyll
leaves of Indocalamus herklotsii M.
