重组人白细胞介素12基因在COS－7细胞的高效表达

High Efficiency Eukaryotic Expression of rhIL-12 in COS-7 Cells

将分别含有重组人ＩＬ－１２（ｒｈＩＬ－１２）两个亚单位ｃＤＮＡ的高效真核细胞表达载体，通过ＤＥＡＥ－Ｄｅｘｔｒａｎ改良方法，共转染至ＣＯＳ－７细胞中。结果高效表达了ｒｈＩＬ－１２蛋白，表达量为６００Ｕ／ｍｌ；并研究其对人ＰＢＭＣ的促增殖作用和增加ＮＫ细胞毒活性的作用，为进一步研究ＩＬ－１２的生物学功能奠定了基础。

In this study,two high-efficiency eukaryotic expression vectors,PED35 and PED40 containing p35 and p40 cDNA of rhIL-12 respectively,were cotransfected into COS-7 cells by modified DEAE-Dextran method.The COS-7 cell culture supernatant had been certified to contain high level of expressed rhIL-12 protein, nearly 600 IU/ml.Furthermore,in the two biological assays,the expressed rhIL- 12 protein can cause the proliferation of human PHA-activated lymphoblasts and augmentation of NK cells mediated cytotoxic activity,This study,therefore,could help further exploration of biological activities and potential usage of rhIL-12.