重组痘苗病毒为载体表达脊髓灰质炎病毒（Ⅰ型）及甲型肝炎病毒双价抗原

Expressing Poliovirus (Type I)and Hepatitis A Virus Antigens by Recombinant Vaccinia Virus

本文报道含有脊髓灰质炎病毒Ⅰ型中和抗原位点及甲型肝炎病毒中和抗原位点重组痘苗病毒的构建，用重组痘苗病毒感染１４３ＴＫ－细胞后成功地表达了脊髓灰质炎病毒Ⅰ型和甲型肝炎病毒的抗原。免疫荧光显示，两种抗原的荧光颗粒均分布在胞浆内。Ｗｅｓｔｅｒｎｂｌｏｔ证实，重组痘苗病毒所表达的脊髓灰质炎病毒Ⅰ型抗原和甲型肝炎病毒抗原为一融合蛋白。用此重组痘苗病毒免疫动物，可诱导脊髓灰质炎病毒Ⅰ型和甲型肝炎病毒的中和抗体产生。

A recombinant vaccinia virus expressing PV-I and HAV antigens was constructed.Dot blot and Southern blot confirmed that the recombinant virus genome contained PV-I cDNA and HAV cDNA chimeric fragment.The result of IF indicated that PV-I antigen and HAV antigen were successfully expressed in the mammalian cells infected with the recombinant virus.The expressed PV-I and HAV antigen showed one band which had both PV-I and HAV antigenicities in Western blot.The band was about 120000(MW).When guinea pigs were immunized with the recombinant virus,PV-I and HAV neutralizing antibodies were induced. The result indicated that the recombinant virus constructed here had bi-valence of PV-I and HAV antigen as well as PV-I and HAV immunogenicities.