表达反义N－myc基因逆转录病毒载体的构建

Construction of a Retroviral Vector Expressing Antisense N-myc Gene

表达反义Ｎ－ｍｙｃ基因逆转录病毒载体的构建陈杰，刘彤华，王志永，崔全才，李和伟，郭洪涛，高洁（中国医学科学院，中国协和医科大学协和医院，北京１００７３０）关键词逆转录病毒载体；Ｎ－ｍｙｃ基因中图号Ｒ７３０．２Ｎ－ｍｙｃ癌基因是ｍｙｃ族癌基因的重要成员...

Abstract In their previous studies the authors demonstrated that nerve growth factor-induced differentiation of neuroblastoma cell lines was closely related with nerve growth factor receptors and amplification of N-myc oncogenes.The authors have also successfully restored the nerve growth factor receptor expression in the cell lines which lacked expression of nerve growth factor receptors.In order to clearify the role of N-myc oncogenes during nerve growth factor-induced differentiation of neuroblastoma cell lines,a new retroviral vector was constructed to express antisense N-myc genes.This new vector contained a puromycin resistant gene,which allowed the authors to express another gene in the cell lines which had already been neomycin resistant.