IS6110-限制性片段长度多态性和间隔区寡核苷酸分型技术在结核分枝杆菌菌株鉴定中的应用

Application of IS6110 Restriction Fragment Length Polymorphism and Spoligotyping in Identification of Mycobacterium Tuberculosis Strains

目的评价IS6110-限制性片段长度多态性（IS6110-RFLP）和间隔区寡核苷酸分型技术（spoligotyping）两种方法在结核病流行病学中的应用，探讨我国各地区的结核分枝杆菌菌株特点。方法收集158株结核分枝杆菌临床分离株，分别应用IS6110-RFLP和Spoligotyping两种方法进行鉴定。结果①IS6110-RFLP的分辨力大于spoligotyping分型。②将本次实验结果与国际spoligotype数据库进行比较。结果有14个类型属于共有类型，其中类型1为流行的类型，分布广泛，即所说的北京基因型。③广东地区与其他地区成簇率和北京基因型所占比例差异有统计学意义（P〈0．05）。广东地区成簇率和北京基因型所占比例均显著低于其他地区。结论同时应用IS6110-RFLP分型和Spoligotyping两种方法进行结核病流行病学调查研究非常有效，在中国不同地区的菌株具有不同的特点。

Objective To assess the application of IS6110-restriction fragment length polymorphism （RFLP）and Spoligotyping in the epidemiology of tuberculosis, and to discuss the characteristic of the Myeobaeterium tuberculosis in different regions in China. Methods A total of 158 Mycobaeterium tuberculosis isolates were identified by IS6110-RFLP and Spoligotyping. Results The discriminatory power of IS6110-RFLP was higher than that of spoligotyping. The result of Spoligotyping was compared with international Spoligotype database. Fourteen types belonged to the shared types, in which the type 1 was epidemic and widely,called Beijing genotype. There was significantly difference among the myeobaeterium tuberculosis between Guangdong and other regions in clustered rate and the proportion of Beijing genotypo （P〈0.05）. The clustered rate and the proportion of Beijing genotype in Guangdong were lower than that in other regions. Conclusion The results of this study indicate that both IS6110-RFLP and Spoligotyping are very useful for the epidemiological investigation of tuberculosis, The Mycobacterium tuberculosis strains in different regions have different character in China.