NZB和NZW小鼠脾淋巴细胞CD48和CD244分子表达及其基因多态性研究

The study of the expression and genetic polymorphism of CD48 and CD244 in the NZB and NZW mice

目的:了解NZB小鼠中是否存在与其CD8+T细胞增殖相关的CD48和CD244分子表达异常及其机制。方法:利用流式细胞分析技术检测NZB和NZW小鼠脾淋巴细胞表面CD48和CD244分子的表达。利用DNA序列测定进行CD48和CD244基因多态性分析。结果:NZB小鼠活化的CD8+T细胞表面CD48和CD244分子表达水平均高于NZW小鼠。DNA序列分析显示CD48和CD244cDNA序列在NZB和NZW小鼠间不存在多态性,但是NZB小鼠的CD48基因转录起始点上游-119bp处存在5个碱基缺失。结论:NZB小鼠活化的脾淋巴细胞高水平表达CD48和CD244分子可能是其CD8+T细胞增生的原因之一。NZBCD48基因转录调控区域异常可能与其高水平表达CD48分子有关。

Objective： To investigate the mechanism of the proliferation of CD8^＋T cell in NZB mice. Methods： Flow eytometry was used to detect the expression of CD48 and CD244 on the surface of spleen lymphocytes from NZB and NZW mice. Single nucleotide polymorphism of CD48 and CD244 gene was determined by DNA sequencing. Results：The level of the expression of CD48 and CD244 on activated CD8^＋T cell of NZB mice was higher than that of NZW mice. No single nucleotide polymorplfism in the eDNA sequences of CD48 and CD244 was found between NZB and NZW mice. Nevertheless, it was found that there was a 5 bp-long deletion at the site of - 119 bp upstream the transcription starting site of CD48 gene. Condusion： High expression of CD48 and CD244 on activated lymphocyte may account for the proliferation of CD8^＋T cell of NZB mice since the interaction of CD48/CD244 regulates the proliferation of CD8^＋T cell. The aberration in the transeription regulatory region of CD48 gene may associate with the high expression of CD48 in NZB mice.