吴茱萸炮制品中总黄酮的含量及其清除羟自由基的作用研究

Contens and scavenging effect on hydroxyl radical of flavonoids in Evodia rutaecarpa

目的测定中药吴茱萸及其炮制品中黄酮类成分的含量;研究其黄酮类成分清除羟自由基的作用。方法以硝酸铝亚硝酸钠分光光度法测定总黄酮含量;利用fenton反应产生羟自由基,水杨酸钠捕捉羟自由基而显色,于510nm测定吸光度,研究吴茱萸黄酮类成分消除羟自由基的作用。结果吴茱萸不同炮制品中总黄酮的含量为29.39mg/g～59.64mg/g;吴茱萸总黄酮有较强的清除羟自由基的作用。结论吴茱萸不同炮制品中总黄酮的含量不同,清除羟自由基的作用也有差异。

ABSTRACT ： AIM ： To determine the contents of flavonoids and its scavenging effect of flavonoids in Evodia rutaecarpa on hydroxyl radical. METHODS： The flavonoids were determined by Al（NO3）3-NaNO2 spectrophotography method, with sodium salicylate captured the hydroxyl radical based on Fenton reaction to make development, its absorbance was measured at 510 nm and adopted as clearance of scavenging effect on hydroxyl radical. RESULTS ： The contents of flavonoids were within 29.39 mg· g^- 1-59.64 mg · g^- 1 from Evodia rutaecarpa and its different processed product. CONCLUSION： The flavonoids in Evodia rutaecarpa showed better effect on scavenging hydroxyl radical.