期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Syk mRNA在浸润性乳腺癌组织中的表达及其临床意义 被引量：2

Clinical significance of Syk gene mRNA expression in invasive breast carcinoma tissue
下载PDF
导出
摘要 目的探讨浸润性乳腺癌组织中Syk mRAN的表达及其临床意义。方法采用逆转录聚合酶链反应(RT-PCR)法检测30例乳腺癌患者瘤体及癌旁正常组织中Syk mRAN的含量,同时采用免疫组化SP法检测瘤体石蜡切片中ER、PR的定性表达情况。结果瘤体组织中Syk mRAN的表达水平明显低于癌旁正常组织(P<0.01),但与ER、PR以及临床分期、病理学分级、淋巴结转移等临床病理因素均无关(P>0.05)。结论Syk mRAN的异常表达与乳腺肿瘤的发生有关。 Objective To explore the clinical significance of Syk （splean tyrosine kinase） mRNA expression in invasive breast carcinoma tissues. Methods The contents of Syk mRNA in the breast cancer tissue and non-cancerous tissue were detected by RTPCR in 30 breast carcinoma patients, and ER and PR of the cancer tissue were examined by immunohistochemical SP method. Results There were significant differences in the expression of Syk mRNA between the malignant and non-cancerous breast tissues（P〈0.01 ）, however, we did not find any correlation between reduction/loss of Syk mRNA expression in breast cancer tissue and clinicopathologic factors or other prognostic markers in our series study（P〈0.05）. Conclusion The aberrant expression of Syk mRAN in breast probably plays an important role in the malignant growth of the cells.
作者 齐义新 胡洁 宋振川 董青 单保恩
机构地区 河北医科大学第四医院外科
出处 《肿瘤》 CAS CSCD 北大核心 2005年第5期475-477,共3页 Tumor
基金 河北省科技攻关项目(编号:02276103D-18)
关键词 乳腺肿瘤 SYK MRNA 蛋白质酪氨酸激酶 逆转录酶聚合酶链反应 Breast neoplasms Syk mRNA Protein-tyrosine kinase Reverse transcriptase polymerase chain reaction
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献6

  • 1Turner M, Schweighoffer E,Colucei F, et al. Tyrosine kinase Syk, essential functions for immunoreceptor signaling[J]. Immunol Today,2000, 21: 148-154.
  • 2Tatsuya Toyama, Hirotake Lwase, Hiroko Yamashita, et al. Reduced expression of the Syk gene is correlated with poor prognosis in human breast cancer[J]. Science, 2003,189:97-102.
  • 3Fluck M, Zurcher G, Andres AC,et al. Molecular characterization of the murine Syk protein tyrosine kinase cDNA, transcripts and protein[J]. Biochem Biophys Res Commun,1995,213 : 273-281.
  • 4Brunner N, Thompson EW,Spang-Thomsen M, et al. LacZ transduced human breast cancer xenografts as an in vivo model for the study of invasion and metastasis[J]. Eur J Cancer,1992,28A(12) : 1989-1995.
  • 5Jiang A, Craxton A, Kurosaki T, et al. Different protein tyrosine kinases are required for B cell antigen receptor mediated activation of extracellular signal-regulated kinase, c-Jun NH2-terminal kinase 1, and p38 mitogen-activated protein kinase[J]. J Exp Med, 1998,188:1297-1306.
  • 6Coopman PJP,Domt H, Barth M, et al. The Syk tyrosine kinase suppresses malignant growth of human breast cancer cells[J]. Nature, 2000, 406 : 742-747.

同被引文献23

  • 1Toyama T,Iwase H,Yamashita H,et al.Reduced expression of the Syk gene is correlated with poor prognosis in human breast cancer[J].Cancer Lett,2003,189(1):97-102.
  • 2Yuan Y,Mendez R,Sahin A,et al.Hypermethylation leads to silencing of the Syk gene in human breast cancer[J].Cancer Res,2001,61(14):5558-5561.
  • 3Kochetkova M,McKenzie OL,Bais AJ,et al.CBFA2T3 (MTG16) is a putative breast tumor suppressor gene from the breast cancer loss of heterozygosity region at 16q24.3[J].Cancer Res,2002,62(16):4599-4604.
  • 4Wolf I,O'Kelly J,Rubinek T,et al.15-Hydroxyprostaglandin dehydrogenase is a tumor suppressor of human breast cancer[J].Cancer Res,2006,66(15):7818-7823.
  • 5Kumar R,Neilsen PM,Crawford J,et al.FBXO31 is the chromosome 16q24.3 senescence gene,a candidate breast tumor suppressor,and a component of an SCF complex[J].Cancer Res,2005,65(24):11304-11313.
  • 6Bednarek AK,Keck-Waggoner CL,Daniel RL,et al.WWOX,the FRA16D gene,behaves as a suppressor of tumor growth[J].Cancer Res,2001,61(22):8068-8073.
  • 7Nunez MI,Ludes-Meyers J,Abba MC,et al.Frequent loss of WWOX expression in breast cancer:correlation with estrogen receptor status[J].Breast Cancer Res Treat,2005,89(2):99-105.
  • 8Krop I,Maguire P,Lahti-Domenici J,et al.HIN-1,a putative cytokine highly expressed in normal but not cancerous mammary epithelial cells[J].Proc Natl Acad Sci USA,2001,98(17):9796-9801.
  • 9Krop I,Parker MT,Bloushtain-Qimron N,et al.HIN-1,an inhibitor of cell growth,invasion,and AKT activation[J].Cancer Res,2005,65(21):9659-9669.
  • 10Stewart ZA,Pietenpol JA.Syk:a new player in the field of breast cancer[J].Breast Cancer Res,2001,3(1):5-7.

引证文献2

二级引证文献1

肿瘤
2005年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部